Development and Validation of a Novel Leishmania donovani Screening Cascade for High-Throughput Scrteening Using a Novel Axenic Assay with High Predictivity of Leishmanicidal Intracellular Activity.
A novel axenic amastigote Leishmania assay was developed and validated that reports only cytocidal molecules. It was used to screen a diversity-oriented synthesis library and two new chemical series with antileishmanial activity were identified. The new axenic assay has a significantly improved translation to the intracellular assay.
Development and Validation of a Novel Leishmania donovani Screening Cascade ... - Nühs A, De Rycker M, Manthri S, Comer E, Scherer CA, Schreiber SL, Ioset JR, Gray* DW. PLoS Negl Trop Dis. 2015 Sep 25; 9 9 :e0004094. doi: 10.1371/journal.pntd.0004094.