Expression of Trypanosoma cruzi Topoisomerase II

On going
Start date
Start stage
Chagas disease
Research areas

Dr. Stenio Fragoso, Head of the Laboratory of Molecular Biology of Trypanosomatids at Fundação Oswaldo Cruz (Fiocruz), is interested in testing inhibitors against recombinant T. cruzi Topoisomerase II. To initiate these studies, Dr. Fragoso and investigators from Seattle Children’s Research Institute (SCRI) and the National Institute of Allergy and Infectious Diseases (NIAID)-funded Seattle Structural Genomics Center for Infectious Disease (SSGCID; contract HHSN272201700059C) are collaborating to develop constructs to express and purify T. cruzi Topoisomerase II. Once the protein is purified, it will be incorporated into an in vitro assay to identify new drugs for Chagas disease.