Multiplexed RT-PCR for Diagnosing Febrile Illnesses in Cameroon
Dr. Demanou, Head of the Arbovirus and Hemorrhagic Fever Virus Laboratory at CPC, believed that dengue fever was underestimated in Cameroon, and was interested in determining the causes and prevalence of non-malarial fevers in the country. BVGH connected Dr. Demanou with Dr. Pinsky, who had developed an RT-PCR diagnostic capable of distinguishing between febrile illnesses. Dr. Demanou provided Dr. Pinsky with over 200 plasma and serum samples from Cameroonian febrile patients, which Dr. Pinsky screened using his multiplexed assay. The results indicated that a large proportion of malaria cases were missed by conventional testing, results that could potentially inform diagnostic development for febrile cases in Cameroon.